PrimePCR™ SYBR® Green Assay: CENPJ, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027052

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CENPJ, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CENPJ, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009108
Assay Design:   Intron-spanning
Chromosome Location:   13:25484162-25486171question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000381884 ENST00000545981 ENST00000445729

Gene Information

This gene encodes a protein that belongs to the centromere protein family. During cell division this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway and also as a coactivator of NF-kappaB-mediated transcription likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly a disorder characterized by severely reduced brain size and mental retardation. [provided by RefSeq Jul 2008]

Gene Symbol:   CENPJ
Gene Name:   centromere protein J
Aliases:   BM032, CPAP, LAP, LIP1, MCPH6, MGC131581, MGC131582, MGC142222, MGC142224, SASS4, SCKL4, Sas-4
RefSeq:   NC_000013.10 NG_009165.1 NT_024524.14
Ensembl:   ENSG00000151849
Entrez:   55835
Chromosome Mapping:   13q12.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download