This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027049
PrimePCR™ PreAmp for SYBR® Green Assay: NOS1, Human
PrimePCR™ Template for SYBR® Green Assay: NOS1, Human
The protein encoded by this gene belongs to the family of nitric oxide synthases which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes including neurotransmission and antimicrobial and antitumoral activities. In the brain and peripheral nervous system nitric oxide displays many properties of a neurotransmitter and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases neural regulation of smooth muscle including peristalsis and penile erection. This protein is ubiquitously expressed with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.