PrimePCR™ SYBR® Green Assay: NPHP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027038

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NPHP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPHP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009063
Assay Design:   Intron-spanning
Chromosome Location:   2:110922717-110927430question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000316534 ENST00000445609 ENST00000393272 ENST00000355301 ENST00000417665

Gene Information

This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate and it appears to function in the control of cell division as well as in cell-cell and cell-matrix adhesion signaling likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1 a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1 also referred to as juvenile nephronophthisis with Leber amaurosis which is characterized by kidney and eye disease and with Joubert syndrome type 4 which is characterized by cerebellar ataxia oculomotor apraxia psychomotor delay and neonatal breathing abnormalities sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   NPHP1
Gene Name:   nephronophthisis 1 (juvenile)
Aliases:   FLJ97602, JBTS4, NPH1, SLSN1
RefSeq:   NC_000002.11 NG_008287.1 NT_022135.16
Ensembl:   ENSG00000144061
Entrez:   4867
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download