This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027038
PrimePCR™ PreAmp for SYBR® Green Assay: NPHP1, Human
PrimePCR™ Template for SYBR® Green Assay: NPHP1, Human
This gene encodes a protein with src homology domain 3 (SH3) patterns. This protein interacts with Crk-associated substrate and it appears to function in the control of cell division as well as in cell-cell and cell-matrix adhesion signaling likely as part of a multifunctional complex localized in actin- and microtubule-based structures. Mutations in this gene cause familial juvenile nephronophthisis type 1 a kidney disorder involving both tubules and glomeruli. Defects in this gene are also associated with Senior-Loken syndrome type 1 also referred to as juvenile nephronophthisis with Leber amaurosis which is characterized by kidney and eye disease and with Joubert syndrome type 4 which is characterized by cerebellar ataxia oculomotor apraxia psychomotor delay and neonatal breathing abnormalities sometimes including retinal dystrophy and renal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.