This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027033
PrimePCR™ PreAmp for SYBR® Green Assay: ARNT, Human
PrimePCR™ Template for SYBR® Green Assay: ARNT, Human
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand which includes dioxin and polycyclic aromatic hydrocarbons results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation which results in a TEL-ARNT fusion protein is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.