PrimePCR™ SYBR® Green Assay: SPG20, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009014
Assay Design:   Intron-spanning
Chromosome Location:   13:36905658-36909257question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000438666 ENST00000355182 ENST00000451493 ENST00000423217

Gene Information

This gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with microtubules. Stimulation with epidermal growth factor (EGF) results in protein translocation to the plasma membrane and the protein functions in the degradation and intracellular trafficking of EGF receptor. Multiple alternatively spliced variants encoding the same protein have been identified. Mutations associated with this gene cause autosomal recessive spastic paraplegia 20 (Troyer syndrome). [provided by RefSeq Nov 2008]

Gene Symbol:   SPG20
Gene Name:   spastic paraplegia 20 (Troyer syndrome)
Aliases:   KIAA0610, SPARTIN, TAHCCP1
RefSeq:   NC_000013.10 NG_011559.1 NT_024524.14
Ensembl:   ENSG00000133104
Entrez:   23111
UniGene:   Hs.440414
Chromosome Mapping:   13q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.190000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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