PrimePCR™ SYBR® Green Assay: FBXL13, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027015

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009002
Assay Design:   Intron-spanning
Chromosome Location:   7:102524661-102553599question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000379305 ENST00000436908 ENST00000313221 ENST00000455112 ENST00000393772 ENST00000379308

Gene Information

Members of the F-box protein family such as FBXL13 are characterized by an approximately 40-amino acid F-box motif. SCF complexes formed by SKP1 (MIM 601434) cullin (see CUL1; MIM 603134) and F-box proteins act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box and they interact with ubiquitination targets through other protein interaction domains (Jin et al. 2004 [PubMed 15520277]).[supplied by OMIM Mar 2008]

Gene Symbol:   FBXL13
Gene Name:   F-box and leucine-rich repeat protein 13
Aliases:   FLJ38068, Fbl13, MGC21636
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000161040
Entrez:   222235
UniGene:   Hs.660029
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.650000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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