This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027012
PrimePCR™ PreAmp for SYBR® Green Assay: NR2C2, Human
PrimePCR™ Template for SYBR® Green Assay: NR2C2, Human
Members of the nuclear hormone receptor family such as NR2C2 act as ligand-activated transcription factors. The proteins have an N-terminal transactivation domain a central DNA-binding domain with 2 zinc fingers and a ligand-binding domain at the C terminus. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes (Yoshikawa et al. 1996 [PubMed 8661150]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.