This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026985
PrimePCR™ PreAmp for SYBR® Green Assay: ARID2, Human
PrimePCR™ Template for SYBR® Green Assay: ARID2, Human
ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083) which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al. 2005 [PubMed 15985610]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.