PrimePCR™ SYBR® Green Assay: GINS4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026984

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GINS4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GINS4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008895
Assay Design:   Intron-spanning
Chromosome Location:   8:41387779-41394714question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000520710 ENST00000518671 ENST00000523277 ENST00000276533

Gene Information

The yeast heterotetrameric GINS complex is made up of Sld5 Psf1 (GINS1; MIM 610608) Psf2 (GINS2; MIM 610609) and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al. 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM Mar 2008]

Gene Symbol:   GINS4
Gene Name:   GINS complex subunit 4 (Sld5 homolog)
Aliases:   MGC14799, SLD5
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000147536
Entrez:   84296
Chromosome Mapping:   8p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.570000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download