This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ENOX1, Human
PrimePCR™ Template for SYBR® Green Assay: ENOX1, Human
Electron transport pathways are generally associated with mitochondrial membranes but non-mitochondrial pathways are also biologically significant. Plasma membrane electron transport pathways are involved in functions as diverse as cellular defense intracellular redox homeostasis and control of cell growth and survival. Members of the ecto-NOX family such as CNOX or ENOX1 are involved in plasma membrane transport pathways. These enzymes exhibit both a hydroquinone (NADH) oxidase activity and a protein disulfide-thiol interchange activity in series with each activity cycling every 22 to 26 minutes (Scarlett et al. 2005 [PubMed 15882838]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.