This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026970
PrimePCR™ PreAmp for SYBR® Green Assay: BRWD3, Human
PrimePCR™ Template for SYBR® Green Assay: BRWD3, Human
The protein encoded by this gene contains a bromodomain and several WD repeats. It is thought to have a chromatin-modifying function and may thus play a role in transcription. Mutations in this gene cause mental retardation X-linked type 93 which is also referred to as mental retardation X-linked with macrocephaly. This gene is also associated with translocations in patients with B-cell chronic lymphocytic leukemia. [provided by RefSeq May 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.