PrimePCR™ SYBR® Green Assay: SERPINA7, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008839
Assay Design:   Intron-spanning
Chromosome Location:   X:105279325-105280552question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000327674 ENST00000372563

Gene Information

There are three proteins including thyroxine-binding globulin (TBG) transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 353 -triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein TBG in serum. It belongs to the serpin family in genomics but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency which has been classified as partial deficiency complete deficiency and excess based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found but the full-length nature of these variants has not been determined.[provided by RefSeq May 2010]

Gene Symbol:   SERPINA7
Gene Name:   serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
Aliases:   FLJ17820, TBG
RefSeq:   NC_000023.10 NG_021252.1 NT_011651.17
Ensembl:   ENSG00000123561
Entrez:   6906
UniGene:   Hs.76838
Chromosome Mapping:   Xq22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 34.580000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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