This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MNDA, Human
PrimePCR™ Template for SYBR® Green Assay: MNDA, Human
The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes designated Ifi-201 Ifi-202 and Ifi-203 that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA which contains an interferon-stimulated response element in the 5-prime untranslated region was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2200 kb of FCER1A APCS CRP and SPTA1. In its pattern of expression and/or regulation MNDA resembles IFI16 suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.