PrimePCR™ SYBR® Green Assay: MNDA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008800
Assay Design:   Intron-spanning
Chromosome Location:   1:158801308-158812047question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000368141

Gene Information

The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes designated Ifi-201 Ifi-202 and Ifi-203 that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA which contains an interferon-stimulated response element in the 5-prime untranslated region was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2200 kb of FCER1A APCS CRP and SPTA1. In its pattern of expression and/or regulation MNDA resembles IFI16 suggesting that these genes participate in blood cell-specific responses to interferons. [provided by RefSeq Jul 2008]

Gene Symbol:   MNDA
Gene Name:   myeloid cell nuclear differentiation antigen
Aliases:   PYHIN3
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163563
Entrez:   4332
UniGene:   Hs.153837
Chromosome Mapping:   1q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.540000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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