This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: PSG3, Human
PrimePCR™ Template for SYBR® Green Assay: PSG3, Human
The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily of genes. Members of the CEA family consist of a single N domain with structural similarity to the immunoglobulin variable domains followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif which has been shown to function as an adhesion recognition signal for several integrins in the N-terminal domain (summary by Teglund et al. 1994 [PubMed 7851896]). For additional general information about the PSG gene family see PSG1 (MIM 176390).[supplied by OMIM Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.