This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: FHIT, Human
PrimePCR™ Template for SYBR® Green Assay: FHIT, Human
This gene a member of the histidine triad gene family encodes a diadenosine 5'5'''-P1P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3 where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact aberrant transcripts from this gene have been found in about half of all esophageal stomach and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Oct 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.