PrimePCR™ SYBR® Green Assay: FHIT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008755
Assay Design:   Intron-spanning
Chromosome Location:   3:59737985-59908109question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000492590 ENST00000476844 ENST00000468189 ENST00000341848

Gene Information

This gene a member of the histidine triad gene family encodes a diadenosine 5'5'''-P1P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3 where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact aberrant transcripts from this gene have been found in about half of all esophageal stomach and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Oct 2009]

Gene Symbol:   FHIT
Gene Name:   fragile histidine triad gene
Aliases:   AP3Aase, FRA3B
RefSeq:   NC_000003.11 NT_022517.18 NG_007551.1
Ensembl:   ENSG00000189283
Entrez:   2272
UniGene:   Hs.655995
Chromosome Mapping:   3p14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.780000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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