PrimePCR™ SYBR® Green Assay: EFR3A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026918

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EFR3A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EFR3A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008704
Assay Design:   Intron-spanning
Chromosome Location:   8:133014918-133023091question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000377917 ENST00000254624 ENST00000407309 ENST00000334503 ENST00000519656

Gene Information

This gene encodes a membrane protein. Studies with orthologous gene in mouse show that it is differentially expressed in the auditory brainstem neurons of mice with hearing deficit compared to mice with normal hearing ability suggesting a role for this gene in hearing. [provided by RefSeq Jan 2011]

Gene Symbol:   EFR3A
Gene Name:   EFR3 homolog A (S. cerevisiae)
Aliases:   DKFZp781J0562, KIAA0143
RefSeq:   NC_000008.10 NG_028112.1 NT_008046.16
Ensembl:   ENSG00000132294
Entrez:   23167
Chromosome Mapping:   8q24.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.810000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download