PrimePCR™ SYBR® Green Assay: PRIM1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008561
Assay Design:   Intron-spanning
Chromosome Location:   12:57140710-57144854question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000338193 ENST00000550770 ENST00000537418

Gene Information

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus in which DNA polymerase alpha and primase are two key enzymatic components. Primase which is a heterodimer of a small subunit and a large subunit synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small 49 kDa primase subunit. [provided by RefSeq Jul 2008]

Gene Symbol:   PRIM1
Gene Name:   primase, DNA, polypeptide 1 (49kDa)
Aliases:   MGC12308, p49
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000198056
Entrez:   5557
UniGene:   Hs.534339
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 34.670000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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