This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: GPR176, Human
PrimePCR™ Template for SYBR® Green Assay: GPR176, Human
Members of the G protein-coupled receptor family such as GPR176 are cell surface receptors involved in responses to hormones growth factors and neurotransmitters (Hata et al. 1995 [PubMed 7893747]).[supplied by OMIM Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.