PrimePCR™ SYBR® Green Assay: PHF6, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008303
Assay Design:   Intron-spanning
Chromosome Location:   X:133547864-133549082question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000370803 ENST00000332070 ENST00000370799 ENST00000370800 ENST00000394292 ENST00000416404

Gene Information

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains indicating a potential role in transcriptional regulation that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS) a disorder characterized by mental retardation epilepsy hypogonadism hypometabolism obesity swelling of subcutaneous tissue of the face narrow palpebral fissures and large ears. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jun 2010]

Gene Symbol:   PHF6
Gene Name:   PHD finger protein 6
Aliases:   BFLS, BORJ, MGC14797
RefSeq:   NC_000023.10 NG_008886.1 NT_011786.16
Ensembl:   ENSG00000156531
Entrez:   84295
UniGene:   Hs.356501
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.990000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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