This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: VPS25, Human
PrimePCR™ Template for SYBR® Green Assay: VPS25, Human
VPS25 VPS36 (MIM 610903) and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II) a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25 VPS36 and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al. 2005 [PubMed 15755741]; Kamura et al. 2001 [PubMed 11278625]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.