PrimePCR™ SYBR® Green Assay: RFXAP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008128
Assay Design:   Intron-spanning
Chromosome Location:   13:37399565-37401835question
Amplicon Length:   134
Splice Variants Targeted:   ENST00000255476

Gene Information

Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5 forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter this complex associates with the non-DNA-binding factor MHC class II transactivator which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RFXAP
Gene Name:   regulatory factor X-associated protein
Aliases:   Not Available
RefSeq:   NC_000013.10 NG_007876.1 NT_024524.14
Ensembl:   ENSG00000133111
Entrez:   5994
UniGene:   Hs.24422
Chromosome Mapping:   13q14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.870000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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