This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026638
PrimePCR™ PreAmp for SYBR® Green Assay: COPZ2, Human
PrimePCR™ Template for SYBR® Green Assay: COPZ2, Human
This gene encodes a subunit of the coatomer protein complex a seven-subunit complex that functions in the formation of COPI-type non-clathrin-coated vesicles. COPI vesicles function in the retrograde Golgi-to-ER transport of dilysine-tagged proteins. This gene is similar to a related family member and the two encoded proteins form distinct isotypes of the coatomer protein complex. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.