This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026613
PrimePCR™ PreAmp for SYBR® Green Assay: EGFL6, Human
PrimePCR™ Template for SYBR® Green Assay: EGFL6, Human
This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle proliferation and developmental processes. The gene product contains a signal peptide suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain which is believed to have an adhesive function. This gene is expressed early during development and its expression has been detected in lung and meningioma tumors. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.