This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: GRP, Human
PrimePCR™ Template for SYBR® Green Assay: GRP, Human
This gene encodes a member of the bombesin-like family of gastrin-releasing peptides. Its preproprotein following cleavage of a signal peptide is further processed to produce either the 27 aa gastrin-releasing peptide or the 10 aa neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems including release of gastrointestinal hormones smooth muscle cell contraction and epithelial cell proliferation. These peptides are also likely to play a role in human cancers of the lung colon stomach pancreas breast and prostate. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.