This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NFYA, Human
PrimePCR™ Template for SYBR® Green Assay: NFYA, Human
The protein encoded by this gene is one subunit of a trimeric complex forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit suggesting a role as the regulatory subunit. In addition there is evidence of post-transcriptional regulation in this gene product either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain with clear tissue-specific preferences for the two isoforms. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.