This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026522
PrimePCR™ PreAmp for SYBR® Green Assay: SLC22A23, Human
PrimePCR™ Template for SYBR® Green Assay: SLC22A23, Human
SLC22A23 belongs to a large family of transmembrane proteins that function as uniporters symporters and antiporters to transport organic ions across cell membranes (Jacobsson et al. 2007 [PubMed 17714910]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.