PrimePCR™ SYBR® Green Assay: RAD21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: RAD21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RAD21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007511
Assay Design:   Intron-spanning
Chromosome Location:   8:117875449-117878867question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000297338 ENST00000520992 ENST00000517485 ENST00000519837 ENST00000522699

Gene Information

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21 a gene involved in the repair of DNA double-strand breaks as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD21
Gene Name:   RAD21 homolog (S. pombe)
Aliases:   FLJ25655, FLJ40596, HR21, HRAD21, KIAA0078, MCD1, NXP1, SCC1, hHR21
RefSeq:   NC_000008.10 NT_008046.16
Ensembl:   ENSG00000164754
Entrez:   5885
Chromosome Mapping:   8q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download