This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026452
PrimePCR™ PreAmp for SYBR® Green Assay: EYA1, Human
PrimePCR™ Template for SYBR® Green Assay: EYA1, Human
This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney branchial arches eye and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome branchiootic syndrome and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.