PrimePCR™ SYBR® Green Assay: EYA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026452

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: EYA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: EYA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007392
Assay Design:   Intron-spanning
Chromosome Location:   8:72184049-72211365question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000388742 ENST00000340726 ENST00000303824 ENST00000388741 ENST00000388743 ENST00000419131 ENST00000388740 ENST00000388744

Gene Information

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney branchial arches eye and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome branchiootic syndrome and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   EYA1
Gene Name:   eyes absent homolog 1 (Drosophila)
Aliases:   BOP, BOR, MGC141875
RefSeq:   NC_000008.10 NG_011735.1 NT_008183.19
Ensembl:   ENSG00000104313
Entrez:   2138
Chromosome Mapping:   8q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.580000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download