PrimePCR™ SYBR® Green Assay: ILVBL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007300
Assay Design:   Intron-spanning
Chromosome Location:   19:15230838-15233576question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000263383 ENST00000527093

Gene Information

The protein encoded by this gene shares similarity with several thiamine pyrophosphate-binding proteins identified in bacteria yeast and plants. The highest degree of similarity is found with bacterial acetolactate synthases (AHAS) which are enzymes that catalyze the first step in branched-chain amino acid biosynthesis. [provided by RefSeq Jul 2008]

Gene Symbol:   ILVBL
Gene Name:   ilvB (bacterial acetolactate synthase)-like
Aliases:   209L8, AHAS, ILV2H, MGC1269, MGC19535
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000105135
Entrez:   10994
UniGene:   Hs.78880
Chromosome Mapping:   19p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 34.530000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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