This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best efficiency
PrimePCR™ PreAmp for SYBR® Green Assay: POLR2G, Human
PrimePCR™ Template for SYBR® Green Assay: POLR2G, Human
This gene encodes the seventh largest subunit of RNA polymerase II the polymerase responsible for synthesizing messenger RNA in eukaryotes. The protein functions in transcription initiation and is also thought to help stabilize transcribing polyermase molecules during elongation. [provided by RefSeq Jan 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.