This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026398
PrimePCR™ PreAmp for SYBR® Green Assay: VCP, Human
PrimePCR™ Template for SYBR® Green Assay: VCP, Human
The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion 26S proteasome function and assembly of peroxisomes. This protein as a structural protein is associated with clathrin and heat-shock protein Hsc70 to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis including homotypic membrane fusion spindle pole body function and ubiquitin-dependent protein degradation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.