This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026384
PrimePCR™ PreAmp for SYBR® Green Assay: FUCA2, Human
PrimePCR™ Template for SYBR® Green Assay: FUCA2, Human
This gene encodes a plasma alpha-L-fucosidase which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family and catalyzes the hydrolysis of the alpha-16-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq Aug 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.