PrimePCR™ SYBR® Green Assay: CLNK, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CLNK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CLNK, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007177
Assay Design:   Intron-spanning
Chromosome Location:   4:10566330-10567711question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000226951 ENST00000507719 ENST00000429087 ENST00000442825

Gene Information

MIST is a member of the SLP76 family of adaptors (see LCP2 MIM 601603; BLNK MIM 604515). MIST plays a role in the regulation of immunoreceptor signaling including PLC-gamma (PLCG1; MIM 172420)-mediated B cell antigen receptor (BCR) signaling and FC-epsilon R1 (see FCER1A MIM 147140)-mediated mast cell degranulation (Cao et al. 1999 [PubMed 10562326]; Goitsuka et al. 2000 2001 [PubMed 10744659] [PubMed 11463797]).[supplied by OMIM Mar 2008]

Gene Symbol:   CLNK
Gene Name:   cytokine-dependent hematopoietic cell linker
Aliases:   MGC35197, MIST
RefSeq:   NC_000004.11 NT_006316.16
Ensembl:   ENSG00000109684
Entrez:   116449
Chromosome Mapping:   4p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download