PrimePCR™ SYBR® Green Assay: DNAH7, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007116
Assay Design:   Intron-spanning
Chromosome Location:   2:196892729-196912172question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000312428 ENST00000410072 ENST00000427816 ENST00000312446

Gene Information

DNAH7 is a component of the inner dynein arm of ciliary axonemes (Zhang et al. 2002 [PubMed 11877439]).[supplied by OMIM Mar 2008]

Gene Symbol:   DNAH7
Gene Name:   dynein, axonemal, heavy chain 7
Aliases:   DKFZp686C09101, FLJ37196, KIAA0944, MGC39580
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000118997
Entrez:   56171
UniGene:   Hs.97403
Chromosome Mapping:   2q32.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.100000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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