PrimePCR™ SYBR® Green Assay: CAB39L, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007109
Assay Design:   Intron-spanning
Chromosome Location:   13:49933885-49951181question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000355854 ENST00000409308 ENST00000425242 ENST00000410043 ENST00000457041 ENST00000413278 ENST00000347776 ENST00000378341

Gene Information

Description Not Available

Gene Symbol:   CAB39L
Gene Name:   calcium binding protein 39-like
Aliases:   FLJ12577, MO25-BETA, MO2L, RP11-103J18.3, bA103J18.3
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000102547
Entrez:   81617
UniGene:   Hs.87159
Chromosome Mapping:   13q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.500000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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