PrimePCR™ SYBR® Green Assay: MPP6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026347

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: MPP6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MPP6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007096
Assay Design:   Intron-spanning
Chromosome Location:   7:24681435-24689309question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000432190 ENST00000222644 ENST00000409761 ENST00000396475 ENST00000430180

Gene Information

Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane cytoskeletal and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies DLG-like (see DLG1; MIM 601014) ZO1-like (see TJP1; MIM 601009) p55-like (see MPP1; MIM 305360) and LIN2-like (see CASK; MIM 300172) based on their size and the presence of additional domains. MPP6 is a member of the p55-like MAGUK subfamily (Tseng et al. 2001 [PubMed 11311936]).[supplied by OMIM Mar 2008]

Gene Symbol:   MPP6
Gene Name:   membrane protein, palmitoylated 6 (MAGUK p55 subfamily member 6)
Aliases:   PALS2, VAM-1, VAM1, p55T
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000105926
Entrez:   51678
Chromosome Mapping:   7p15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 34.720000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download