This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026334
PrimePCR™ PreAmp for SYBR® Green Assay: ADD3, Human
PrimePCR™ Template for SYBR® Green Assay: ADD3, Human
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin originally purified from human erythrocytes was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C the binding site for calmodulin and is required for association with spectrin and actin. Alternatively spliced adducin gamma transcripts encoding different isoforms have been described. The functions of the different isoforms are not known. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.