This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: WRAP53, Human
PrimePCR™ Template for SYBR® Green Assay: WRAP53, Human
This gene encodes an essential component of the telomerase holoenzyme complex a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin TERT and TERC other components of active telomerase and with small Cajal body RNAs (scaRNAs) which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.