PrimePCR™ SYBR® Green Assay: WRAP53, Human

print
PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006849
Assay Design:   Intron-spanning
Chromosome Location:   17:7592908-7604115question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000431639 ENST00000316024 ENST00000457584 ENST00000396463 ENST00000534050

Gene Information

This gene encodes an essential component of the telomerase holoenzyme complex a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin TERT and TERC other components of active telomerase and with small Cajal body RNAs (scaRNAs) which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   WRAP53
Gene Name:   WD repeat containing, antisense to TP53
Aliases:   FLJ10385, TCAB1, WDR79
RefSeq:   NC_000017.10 NG_017013.1 NT_010718.16
Ensembl:   ENSG00000141499
Entrez:   55135
UniGene:   Hs.408312
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 34.460000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
Get Help

Call us at 1-800-4-BIORAD (1-800-424-6723)

Sign Up for Bio-Rad Updates!
Enter your email address below to receive your choice of the latest news, promotions, and more.