PrimePCR™ SYBR® Green Assay: WRAP53, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: WRAP53, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WRAP53, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006849
Assay Design:   Intron-spanning
Chromosome Location:   17:7592908-7604115question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000431639 ENST00000316024 ENST00000457584 ENST00000396463 ENST00000534050

Gene Information

This gene encodes an essential component of the telomerase holoenzyme complex a ribonucleoprotein complex required for telomere synthesis. This protein is enriched in Cajal bodies nuclear sites of RNP processing that are important for telomerase function. It interacts with dyskerin TERT and TERC other components of active telomerase and with small Cajal body RNAs (scaRNAs) which are involved in modifying splicing RNAs. This mRNA also functions as a p53 antisense transcript that regulates endogenous p53 mRNA levels and further induction of p53 protein by targeting the 5' untranslated region of p53 mRNA. Alternatively spliced transcript variants which differ only in the 5' UTR have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   WRAP53
Gene Name:   WD repeat containing, antisense to TP53
Aliases:   FLJ10385, TCAB1, WDR79
RefSeq:   NC_000017.10 NG_017013.1 NT_010718.16
Ensembl:   ENSG00000141499
Entrez:   55135
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996200
y-intercept 34.460000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download