This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026310
PrimePCR™ PreAmp for SYBR® Green Assay: GCA, Human
PrimePCR™ Template for SYBR® Green Assay: GCA, Human
This gene product grancalcin is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin calpain and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation grancalcin localizes to the cytosolic fraction; with magnesium alone it partitions with the granule fraction; and in the presence of magnesium and calcium it associates with both the granule and membrane fractions suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.