PrimePCR™ SYBR® Green Assay: JAG1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006831
Assay Design:   Intron-spanning
Chromosome Location:   20:10623213-10625011question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000254958 ENST00000423891

Gene Information

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1 the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq Jul 2008]

Gene Symbol:   JAG1
Gene Name:   jagged 1
Aliases:   AGS, AHD, AWS, CD339, HJ1, JAGL1, MGC104644
RefSeq:   NC_000020.10 NG_007496.1 NT_011387.8
Ensembl:   ENSG00000101384
Entrez:   182
UniGene:   Hs.224012
Chromosome Mapping:   20p12.1-p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.010000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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