This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026307
PrimePCR™ PreAmp for SYBR® Green Assay: LPAR1, Human
PrimePCR™ Template for SYBR® Green Assay: LPAR1, Human
The integral membrane protein encoded by this gene is a lysophosphatidic acid (LPA) receptor from a group known as EDG receptors. These receptors are members of the G protein-coupled receptor superfamily. Utilized by LPA for cell signaling EDG receptors mediate diverse biologic functions including proliferation platelet aggregation smooth muscle contraction inhibition of neuroblastoma cell differentiation chemotaxis and tumor cell invasion. Two transcript variants encoding the same protein have been identified for this gene [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.