This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ARNTL2, Human
PrimePCR™ Template for SYBR® Green Assay: ARNTL2, Human
This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER ARNT SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels exposure to certain environmental pollutants and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein the structurally related MOP4 and hypoxia-inducible factors such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors this protein is coexpressed in regions of the brain such as the thalamus hypothalamus and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.