PrimePCR™ SYBR® Green Assay: ARNTL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ARNTL2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARNTL2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006805
Assay Design:   Intron-spanning
Chromosome Location:   12:27543043-27553515question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000544915 ENST00000395901 ENST00000546179 ENST00000311001 ENST00000261178 ENST00000266503 ENST00000457040 ENST00000542388

Gene Information

This gene encodes a basic helix-loop-helix transcription factor belonging to the PAS (PER ARNT SIM) superfamily. The PAS proteins play important roles in adaptation to low atmospheric and cellular oxygen levels exposure to certain environmental pollutants and diurnal oscillations in light and temperature. This protein forms a transcriptionally active heterodimer with the circadian CLOCK protein the structurally related MOP4 and hypoxia-inducible factors such as HIF1alpha. Consistent with its role as a biologically relevant partner of circadian and hypoxia factors this protein is coexpressed in regions of the brain such as the thalamus hypothalamus and amygdala. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   ARNTL2
Gene Name:   aryl hydrocarbon receptor nuclear translocator-like 2
Aliases:   BMAL2, CLIF, MGC149671, MGC149672, MOP9, PASD9, bHLHe6
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000029153
Entrez:   56938
Chromosome Mapping:   12p12.2-p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 36.460000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download