PrimePCR™ SYBR® Green Assay: CEP63, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026296

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CEP63, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CEP63, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006800
Assay Design:   Intron-spanning
Chromosome Location:   3:134268980-134270805question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000332047 ENST00000354446 ENST00000383229 ENST00000513612 ENST00000504929 ENST00000514678 ENST00000337090

Gene Information

This gene encodes a protein with six coiled-coil domains. The protein is localized to the centrosome a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. Several alternatively spliced transcript variants have been found but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CEP63
Gene Name:   centrosomal protein 63kDa
Aliases:   FLJ13386, MGC78416
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000182923
Entrez:   80254
Chromosome Mapping:   3q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.050000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download