PrimePCR™ SYBR® Green Assay: CEACAM5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026257

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006696
Assay Design:   Intron-spanning
Chromosome Location:   19:42222195-42223894question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000221992 ENST00000405816 ENST00000398599 ENST00000378181

Gene Information

Members of the CEACAM subfamily including CEACAM5 belong to the CEA gene family. For general information on the CEA gene family see CEACAM1 (MIM 109770).[supplied by OMIM Oct 2009]

Gene Symbol:   CEACAM5
Gene Name:   carcinoembryonic antigen-related cell adhesion molecule 5
Aliases:   CD66e, CEA, DKFZp781M2392
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105388
Entrez:   1048
UniGene:   Hs.709196
Chromosome Mapping:   19q13.1-q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.680000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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