PrimePCR™ SYBR® Green Assay: RP11-428C6.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026250

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-428C6.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-428C6.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006671
Assay Design:   Intron-spanning
Chromosome Location:   5:73183433-73187957question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000296794 ENST00000437974 ENST00000426542 ENST00000296799 ENST00000512883 ENST00000545377 ENST00000513042 ENST00000287898

Gene Information

This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq Apr 2010]

Gene Symbol:   RP11-428C6.1
Gene Name:   Rho-guanine nucleotide exchange factor
Aliases:   DKFZp686P12164, FLJ21817, KIAA1998, RIP2, p190RHOGEF
RefSeq:   NC_000005.9 NG_017198.1 NT_006713.15
Ensembl:   ENSG00000214944
Entrez:   64283
Chromosome Mapping:   5q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.990000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download