This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026212
PrimePCR™ PreAmp for SYBR® Green Assay: TFE3, Human
PrimePCR™ Template for SYBR® Green Assay: TFE3, Human
The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF TFE3 TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3 SMAD3 and LEF-1 and is involved in TGF-beta-induced transcription playing important roles in cell growth proliferation and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas such as PRCC-TFE3 RCC17-TFE3 PSF-TFE3 NonO (p54nrb)-TFE3 and ASPL-TFE3.[provided by RefSeq Dec 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.