PrimePCR™ SYBR® Green Assay: AMMECR1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AMMECR1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AMMECR1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006462
Assay Design:   Intron-spanning
Chromosome Location:   X:109445714-109459808question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000262844 ENST00000372059 ENST00000372057

Gene Information

The exact function of this gene is not known however submicroscopic deletion of the X chromosome including this gene COL4A5 and FACL4 genes result in a contiguous gene deletion syndrome the AMME complex (Alport syndrome mental retardation midface hypoplasia and elliptocytosis). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:   AMMECR1
Gene Name:   Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1
Aliases:   AMMERC1
RefSeq:   NC_000023.10 NT_011651.17 NG_016469.1
Ensembl:   ENSG00000101935
Entrez:   9949
Chromosome Mapping:   Xq22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.480000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download