PrimePCR™ SYBR® Green Assay: DGKH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026174

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DGKH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DGKH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006455
Assay Design:   Intron-spanning
Chromosome Location:   13:42763389-42764634question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000337343 ENST00000261491 ENST00000540693 ENST00000379274 ENST00000536612 ENST00000538674

Gene Information

This gene encodes a member of the diacylglycerol kinase (DGK) enzyme family of proteins specifically the type II DGK subfamily. Members of this family are involved in regulating the intracellular concentrations of diacylglycerol and phosphatidic acid. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   DGKH
Gene Name:   diacylglycerol kinase, eta
Aliases:   DGKeta, DKFZp761I1510
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000102780
Entrez:   160851
Chromosome Mapping:   13q14.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.180000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download