PrimePCR™ SYBR® Green Assay: IL16, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0025976

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0005999
Assay Design:   Intron-spanning
Chromosome Location:   15:81593750-81595953question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000394660 ENST00000302987 ENST00000394652 ENST00000360547 ENST00000355368 ENST00000329842 ENST00000394653 ENST00000394656

Gene Information

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant a modulator of T cell activation and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq Feb 2010]

Gene Symbol:   IL16
Gene Name:   interleukin 16 (lymphocyte chemoattractant factor)
Aliases:   FLJ16806, FLJ42735, FLJ44234, LCF, NIL16, PRIL16, prIL-16
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000172349
Entrez:   3603
UniGene:   Hs.459095
Chromosome Mapping:   15q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.870000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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