PrimePCR™ SYBR® Green Assay: SUMO3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0005932
Assay Design:   Intron-spanning
Chromosome Location:   21:46233979-46237914question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000397898 ENST00000332859 ENST00000411651

Gene Information

SUMO proteins such as SUMO3 and ubiquitin (see MIM 191339) posttranslationally modify numerous cellular proteins and affect their metabolism and function. However unlike ubiquitination which targets proteins for degradation sumoylation participates in a number of cellular processes such as nuclear transport transcriptional regulation apoptosis and protein stability (Su and Li 2002 [PubMed 12383504]).[supplied by OMIM Mar 2008]

Gene Symbol:   SUMO3
Gene Name:   SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
Aliases:   SMT3A, SMT3H1, SUMO-3, Smt3B
RefSeq:   NC_000021.8 NT_011515.12
Ensembl:   ENSG00000184900
Entrez:   6612
UniGene:   Hs.474005
Chromosome Mapping:   21q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 33.400000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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